pucF [2019-08-09 09:55:39]

allantoate amidohydrolase

locus: BSU_32530

pI: 5.36

mw: 45.36 kDa

protein length: 412 aa

gene length: 1239 bp

function: purine utilization

product: allantoate amidohydrolase

essential: no

synonyms: yurH

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

Gene

Coordinates: 3,342,433 → 3,343,671

The protein

Protein family

• [SW|peptidase M20 family] (according to UniProt)

Structure

• [PDB|2IMO] (from ''Escherichia coli k12'', 47% identity, 63% similarity) [Pubmed|17362992]

Expression and Regulation

Operons

• genes: [gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]-[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]
  description: [Pubmed|12029039]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

  regulation

  • induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]

Biological materials

Mutant

• MGNA-A943 (yurH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/943 NBRP B. subtilis, Japan]
• BKE32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
• BKK32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAA

References

• 11344136,12029039